Technical Proceedings of the 2010 NSTI Nanotechnology Conference & Expo - Nanotech 2010
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Nanotech 2010 Vol. 3
Chapter 7: Nano Medical Sciences
Determination of the Electroporation Threshold for pulses from 95 ns to 20 us
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| Authors: | G. Saulis, S. Balevicius, R. Saule, V. Stankevic, N. Zurauskiene |
| Affiliation: | Vytautas Magnus University, LT |
| Pages: | 506 - 509 |
| Keywords: | nanoporation, mouse hepatoma, electropermeabilization |
| Abstract: | The dependences of the fraction of electroporated mouse hepatoma MH-22A cells on the pulse intensity were obtained for the cells exposed to single square-wave electric pulses with the durations from 95 ns to 20 us. The amplitude of an electric pulse was varied from 0.4 to 12 kV/cm. Increasing the intensity of the electric field pulse increased the fraction of electroporated cells. The electric field which leads to a given percentage of electroporation decreased with increasing the pulse length. The dependence of the amplitude of the electric pulse required to electroporate 50% of mouse hepatoma MH-22A cells on the pulse duration was also determined. |
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