Label-free, Fluorescenece Detection of DNA Nanoparticle Scaffolds

Technical Proceedings of the 2010 NSTI Nanotechnology Conference & Expo - Nanotech 2010

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	Nanotech 2010 Vol. 3 Chapter 1: Bio Sensors, Diagnostics & Imaging Label-free, Fluorescenece Detection of DNA Nanoparticle Scaffolds
Authors:	A.J. Kell, S. Tan, I. Charlebois, M. Boissinot, M. LeClerc, B. Simard
Affiliation:	National Research Council, CA
Pages:	1 - 3
Keywords:	DNA, label-free detection, nanoparticle, polymeric transduction
Abstract:	We have developed a nanoparticle-based DNA biosensor that can detect unlabelled ssDNA with good sensitivity (picomolar concentrations). The biosensor makes use of the optical changes of a cationic polymer interacting with ssDNA versus that of hybridized DNA to provide a fluorescence signal only when a specific hybridization event occurs (i.e. only when perfectly complementary DNA is present)
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